Mechanism of filament formation on ssDNA by RecA protein
نویسندگان
چکیده
RecA protein (RecA) forms a nucleoprotein filament composed of a helical protein array that binds to singlestranded DNA (ssDNA), which in its active form is then able to initiate various reactions. A series of studies to date suggest the following roles of the N-terminal domain on filament formation by RecA. 1) The N-terminal domain of RecA is the functional region for protein-protein interactions between contiguous RecA monomers. 2) The N-terminal domain regulates filament formation kinetically through its folding transition. 3) The N-terminal domain is the trigger for filament formation, which searches for ssDNA regions on the DNA and initiates filament formation on ssDNA.
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